RECOMBINATION-MEDIATED PCR-DIRECTED PLASMID CONSTRUCTION IN-VIVO IN YEAST

Authors
OLDENBURG KR VO KT MICHAELIS S PADDON C
Citation
Kr. Oldenburg et al., RECOMBINATION-MEDIATED PCR-DIRECTED PLASMID CONSTRUCTION IN-VIVO IN YEAST, Nucleic acids research, 25(2), 1997, pp. 451-452
Citations number
11
Categorie Soggetti
Biology
Journal title
Nucleic acids research → ACNP
ISSN journal
03051048
Volume
25
Issue
2
Year of publication
1997
Pages
451 - 452
Database
ISI
SICI code
0305-1048(1997)25:2<451:RPPCII>2.0.ZU;2-P
Abstract
We have extended the technique of PCR-directed recombination in Saccha romyces cerevisiae to develop a simple method for plasmid or gene cons truction in the absence of suitable restriction sites. The DNA to be c loned is PCR-amplified with 30-40 bp of homology to a linearized yeast plasmid. Co-transformation into yeast results in homologous recombina tion ata position directed by the PCR oligonucleotides.