INCORPORATION OF SAMPLE STACKING TECHNIQUES INTO THE CAPILLARY ELECTROPHORESIS CF-FAB MASS-SPECTROMETRIC ANALYSIS OF DNA-ADDUCTS

Sample stacking is used to improve the detection limits of capillary z one electrophoresis coupled to continuous flow fast atom bombardment m ass spectrometry for the analysis of DNA adducts. It was found that, w ith stacking, the concentration detection limit of deoxynucleotide add ucts could be improved by as much as 3 orders of magnitude, thereby br inging it into the 10(-8) M range. In addition, the mass spectrometric mass detection limits of a model acetylaminofluorene deoxyguanosine 5 '-monophosphate adduct were found to be in the low picomole range for full scanning and the low femtomole range for multiple reaction monito ring of a selected fragmentation. The techniques have been applied to the analysis of adducts formed in the in vitro reaction of N-acetoxy-N -acetyl-2-aminofluorene with DNA.