Lactic acid bacteria are nonpathogenic bacteria commonly used in food
processing. An evaluation was made of the capacity to concentrate sele
nium in species of Lactobacillus. A selenium concentration of 1 mu g/m
L in the culture medium yielded in a bacterial content of 400 mu g/g d
ry biomass. Dialysis and TCA precipitation experiments of a native int
racellular extract proved that at least 80% of the total selenium is a
ssociated with organic molecules. Seleno-cysteine was identified as th
e only seleno-amino acid present in the intracellular selenoproteins.
This study shows that species of the lactic acid bacteria are able to
concentrate selenium intracellular as seleno-cysteine, which could be
applied in supplementation studies.