MATRIX-ASSISTED LASER-DESORPTION IONIZATION TIME-OF-FLIGHT MASS-SPECTROMETRY OF DNA-PT(II) COMPLEXES

Matrix-assisted laser desorption ionization (MALDI) time-of-flight mas s spectrometry has been used to characterize the reaction products of the 18-mer deoxyribonucleotide d(AACGGTTAACCGTTAATT) with [Pt(NH3)(3)( H2O)](2+) and cis-[Pt(NH3)(2)(H2O)(2)](2+). Characteristic peaks corre sponding to different monofunctional adducts {18-mer + n[Pt(NH3)(3)]} (n = 1, 2, 3 and 4) have been observed with the triamino-monoaqua comp lex, With the diamino-diaqua cis-Pt complex, formation of a chelate {1 8-mer + [Pt(NH3)(2)]} involving two adjacent guanines has been demonst rated, A good correlation between MALDI and polyacrylamide gel electro phoresis results is observed.