ACCUMULATION OF FIBRONECTIN IN ARTICULAR-CARTILAGE EXPLANTS CULTURED WITH TGF-BETA-1 AND FUCOIDAN

Fibronectin is a glycoprotein involved in cell matrix interactions. In osteoarthritis, fibronectin levels in the lesion cartilage are elevat ed up to all-fold above control levels. In these experiments, explants of disease-free cartilage cultured in the presence of a combination o f TGF beta 1 and the sulfated fucopolysaccharide, fucoidan, accumulate d fibronectin at levels comparable to those found in osteoarthritic le sions. TGF beta 1 increased fibronectin synthesis, most of which was r eleased to the medium. The addition of fucoidan favored retention of t he newly synthesized fibronectin within the matrix. The fibronectin wh ich accumulated as a result of these treatments was similar to the fib ronectin in normal and osteoarthritic cartilage with respect to the ED -B+ alternative splice form. No change in the proteoglycan content of the cartilage explants with elevated fibronectin levels was detected. (C) 1995 Academic Press, Inc.