DETECTION OF SERUM ANTIBODY AGAINST BOVINE COCCIDIOSIS BY USING EIMERIA-TENELLA ANTIGEN

Authors
UCHIDA T",HASBULLAH,"NAKAI Y OGIMOTO K
Citation
Y. Uchida T",hasbullah,"nakai et K. Ogimoto, DETECTION OF SERUM ANTIBODY AGAINST BOVINE COCCIDIOSIS BY USING EIMERIA-TENELLA ANTIGEN, Journal of veterinary medical science, 57(1), 1995, pp. 169-171
Citations number
14
Categorie Soggetti
Veterinary Sciences
Journal title
Journal of veterinary medical science → ACNP
ISSN journal
09167250
Volume
57
Issue
1
Year of publication
1995
Pages
169 - 171
Database
ISI
SICI code
0916-7250(1995)57:1<169:DOSAAB>2.0.ZU;2-P
Abstract
Antigens of Eimeria tenella sporulated oocysts were applied to detect serum antibody of calf infected with Eimeria. Polypeptides of E. tenel la oocyst were strongly proved with the infected bovine serum in immun oblotting. The infected serum also showed higher absorbances than that of normal serum in enzyme linked immunosorbent assay (ELISA) using E. tenella oocyst antigen. Suitable conditions for the ELISA were obtain ed by using soluble antigen of E. tenella oocysts at 2.5 mu g/ml and b ovine serum at 200 fold dilution.