TANDEM MASS-SPECTROMETRY OF CARBONIC-ANHYDRASE (29-KDA)

Authors
OCONNOR PB SPEIR JP SENKO MW LITTLE DP MCLAFFERTY FW
Citation
Pb. Oconnor et al., TANDEM MASS-SPECTROMETRY OF CARBONIC-ANHYDRASE (29-KDA), Journal of mass spectrometry., 30(1), 1995, pp. 88-93
Citations number
35
Categorie Soggetti
Chemistry Inorganic & Nuclear",Spectroscopy,Biophysics
Journal title
Journal of mass spectrometry. → ACNP
ISSN journal
10765174
Volume
30
Issue
1
Year of publication
1995
Pages
88 - 93
Database
ISI
SICI code
1076-5174(1995)30:1<88:TMOC(>2.0.ZU;2-8
Abstract
Using electrospray ionization with a Fourier-transform mass spectromet er, MS/MS of molecular ions and seven fragment ions of carbonic anhydr ase (259 amino acids) yields 74 identifiable fragment ions, including 23 amino acid spacings, Such sensitive and specific direct characteriz ation of proteins appears to be complementary to conventional techniqu es including LC/MS/MS sequencing of oligopeptides from protein degrada tion.