NONISOTOPIC METHOD OF QUANTITATIVE PCR AN ALYSIS IN THE DIAGNOSIS OF HIV-INFECTION

A nonisotopic method for the detection of HIV DNA has been developed, based on the use of avidin-biotin complex to identify the products of polymerase chain reaction (PCR). Biotin label integrated in deoxyuridi ne triphosphate (bio-11-dUTP) was incorporated in the structure of amp lified fragments in the course of PCR. The resultant products were ide ntified in hybridization reaction with specific HIV DNA adsorbed on po lystyrene plates. The suggested method was not inferior to the traditi onal radioisotope method in sensitivity and specificity and helped qua ntitatively assess the results of experiments using an objective crite rion - optic density value.