ISOLATION OF HUMAN B-CELLS SPECIFIC TO VI RAL PEPTIDES USING MAGNETICBEADS

Authors
SIDOROVA YV BORISOVA TK ROZEN A
Citation
Yv. Sidorova et al., ISOLATION OF HUMAN B-CELLS SPECIFIC TO VI RAL PEPTIDES USING MAGNETICBEADS, Voprosy virusologii, 39(6), 1994, pp. 272-274
Citations number
12
Categorie Soggetti
Virology
Journal title
Voprosy virusologii → ACNP
ISSN journal
05074088
Volume
39
Issue
6
Year of publication
1994
Pages
272 - 274
Database
ISI
SICI code
0507-4088(1994)39:6<272:IOHBST>2.0.ZU;2-2
Abstract
A method for isolation of human B cells specific to viral peptides usi ng magnetic beads has been developed. The method was used for the isol ation from human tonsils of B cells specific to HIV-I gp 120 MN-24 pep tide (residues 302-322) and to Epstein-Barr virus 107 EBNA-1 peptide. The cells selected with beads were transformed by Epstein-Barr virus. The resultant cell cultures produce human immunoglobulin antibodies to HIV-1 MN-24 and to Epstein-Barr EBNA-1 p107 peptides.