PSEUDOSUSPENSION CULTURING OF CELLS TO OB TAIN ANTIGENS OF INFLUENZA-A AND INFLUENZA-B VIRUSES

Conditions for pseudosuspension culturing of continuous cells of the k idneys of dogs (MDCK), green marmosets (CV-1), and swine (SPEV) on two types of microcarriers (Cytogel-3 and cytolar-2) under semiproduction conditions (50 liter bioreactor) were developed on a model of reassor tants of influenza viruses A and B. To standardize the conditions, nat ive sera were replaced by growth-stimulating proteins isolated from th e sera of various animals (cattle, northern dears, swines). A better a dhesive capacity and more active cell proliferation on porous microcar rier cytogel-3 were observed. The highest proliferative activity of ce lls was observed when porcine growth-stimulating proteins were used. I nfluenza A virus reassortant (H3N1) was actively reproduced in MDCK ce lls. The reproduction of influenza B virus reassortant was similarly h igher in MDCK cells, in comparison with SPEV cells; at the same time, no differences in hemagglutinin titers in the two cell cultures were o bserved. The pseudosuspension method of cell culturing is recommended for the preparation of influenza antigens.