Ry. Podchernyayeva et Va. Isachenko, PSEUDOSUSPENSION CULTURING OF CELLS TO OB TAIN ANTIGENS OF INFLUENZA-A AND INFLUENZA-B VIRUSES, Voprosy virusologii, 39(6), 1994, pp. 274-276
Conditions for pseudosuspension culturing of continuous cells of the k
idneys of dogs (MDCK), green marmosets (CV-1), and swine (SPEV) on two
types of microcarriers (Cytogel-3 and cytolar-2) under semiproduction
conditions (50 liter bioreactor) were developed on a model of reassor
tants of influenza viruses A and B. To standardize the conditions, nat
ive sera were replaced by growth-stimulating proteins isolated from th
e sera of various animals (cattle, northern dears, swines). A better a
dhesive capacity and more active cell proliferation on porous microcar
rier cytogel-3 were observed. The highest proliferative activity of ce
lls was observed when porcine growth-stimulating proteins were used. I
nfluenza A virus reassortant (H3N1) was actively reproduced in MDCK ce
lls. The reproduction of influenza B virus reassortant was similarly h
igher in MDCK cells, in comparison with SPEV cells; at the same time,
no differences in hemagglutinin titers in the two cell cultures were o
bserved. The pseudosuspension method of cell culturing is recommended
for the preparation of influenza antigens.