EFFECT OF FETAL CALF SERUM ON ANTI-HIV AC TIVITY OF MYRAMISTIN

The anti-HIV activity of an antiseptic myramistin in the presence of f etal calf serum (FCS) was studied. It has been recently shown that cat ion detergent myramistin at a concentration of 100 mu g/ml prevented H IV-1 repllication in MT-4 cells when these cells were cocultivated wit h MT-4 cells previously infected and treated with the detergent in a p rotein-free medium. Under such conditions 50 mu g of myramistin per mi delayed HIV propagation in MT-4 cells by 14 days. Detergent-dependent arrest of HIV development was abolished by addition of FCS at a final concentration of 50%. Myramistin in a dose of 100 mu g/ml prevents HI V-1 replication in Jurkat-tat cells when they are cocultivated with th e cells of the same line previously infected and treated by the deterg ent in the presence of 5% FCS. FCS in concentrations from 12.5 to 75% prevents the anti-HIV activity of myramistin in a dose of 100 mu g/ml.