TRANSPLANTATION TO THE RAT-BRAIN OF HUMAN NEURAL PROGENITORS THAT WERE GENETICALLY-MODIFIED USING ADENOVIRUSES

Transplantations for neurological disorders are limited by the supply of human fetal tissue. To generate larger numbers of cells of appropri ate phenotype, we investigated whether human neural progenitors expand ed in vitro could be modified with recombinant adenoviruses. Strong ex pression of beta-galactosidase was obtained in vitro. Two or three wee ks after transplantation of engineered cells to the rat brain, we obse rved a small percentage of surviving neuroblasts strongly expressing b eta-galactosidase in four out of 13 rats. Thus human precursor cells t hat have been genetically modified using adenoviruses are a promising tool for ex vivo gene therapy of neurodegenerative diseases.