P. Prigent et al., MERCURIC CHLORIDE-INDUCED PROGRAMMED CELL-DEATH OF A MURINE T-CELL HYBRIDOMA .2. OPPOSITE EFFECT OF INTERLEUKIN-2 AND INTERLEUKIN-4, Cellular immunology, 161(1), 1995, pp. 107-111
In susceptible animals evidence is accumulating for a primary role for
Th2 cells in the course of HgCl2-induced autoimmunity, and for a cont
ribution of Th1 cells in the self-regulated phase of this disease. We
have reported that incubation of 2B4.11 T cell hybridoma with HgCl2 in
duced programmed cell death. This paper shows that recombinant IL-2 si
gnificantly diminished HgCl2-induced 2B4.11 cell death. Although no ef
fect was observed upon incubation with exogenous IL-4, we observed a s
ignificant protection by adding an anti-IL-4 monoclonal antibody to th
e culture. Accordingly, by RT-PCR we found the presence of IL-2 recept
or-encoding mRNA, and by cytofluorometry, the expression of the protei
n was detected only after exposure to HgCl2. Moreover, upon HgCl2 trea
tment, 2B4.11 cells were induced to produce IL-4. Altogether these fin
dings showed that cytokine environment, IL-2, IL-4 otherwise defining
the Th1/Th2 dichotomy, in conjunction with a chemical may differential
ly influence the fate of cell populations, death or survival. (C) 1995
Academic Press, Inc.