MUTATIONS RESULTING IN A DEFECTIVE MINI-F REPLICON IN THE PRESENCE OFBOTH ORIGINS, ORII AND ORIII

Authors
DISQUEKOCHEM C GERHUS E EICHENLAUB R
Citation
C. Disquekochem et al., MUTATIONS RESULTING IN A DEFECTIVE MINI-F REPLICON IN THE PRESENCE OFBOTH ORIGINS, ORII AND ORIII, Plasmid, 33(1), 1995, pp. 59-64
Citations number
37
Categorie Soggetti
Genetics & Heredity
Journal title
Plasmid → ACNP
ISSN journal
0147619X
Volume
33
Issue
1
Year of publication
1995
Pages
59 - 64
Database
ISI
SICI code
0147-619X(1995)33:1<59:MRIADM>2.0.ZU;2-K
Abstract
Within the region of the origin of replication (oriII) of plasmid mini -F, we introduced a set of deletions around the XhoI site (45.080F) an d an insertion of 4 nucleotides at the BglII site (45.213F). These alt erations had the effect that replication from oriII as well as from th e alternative origin, oriI, was abolished. The analysis suggests that an AT-rich region which may function as entry site for DnaB-DnaC prote in is an essential structural requirement for replication of plasmid m ini-F. (C) 1995 Academic Press, Inc.