C. Disquekochem et al., MUTATIONS RESULTING IN A DEFECTIVE MINI-F REPLICON IN THE PRESENCE OFBOTH ORIGINS, ORII AND ORIII, Plasmid, 33(1), 1995, pp. 59-64
Within the region of the origin of replication (oriII) of plasmid mini
-F, we introduced a set of deletions around the XhoI site (45.080F) an
d an insertion of 4 nucleotides at the BglII site (45.213F). These alt
erations had the effect that replication from oriII as well as from th
e alternative origin, oriI, was abolished. The analysis suggests that
an AT-rich region which may function as entry site for DnaB-DnaC prote
in is an essential structural requirement for replication of plasmid m
ini-F. (C) 1995 Academic Press, Inc.