COMPARISON OF THE KINETICS OF PUUMALA VIRUS-SPECIFIC IGM AND IGG ANTIBODY-RESPONSES IN NEPHROPATHIA-EPIDEMICA AS MEASURED BY A RECOMBINANT ANTIGEN-BASED ENZYME-LINKED-IMMUNOSORBENT-ASSAY AND AN IMMUNOFLUORESCENCE TEST
F. Elgh et al., COMPARISON OF THE KINETICS OF PUUMALA VIRUS-SPECIFIC IGM AND IGG ANTIBODY-RESPONSES IN NEPHROPATHIA-EPIDEMICA AS MEASURED BY A RECOMBINANT ANTIGEN-BASED ENZYME-LINKED-IMMUNOSORBENT-ASSAY AND AN IMMUNOFLUORESCENCE TEST, Journal of medical virology, 45(2), 1995, pp. 146-150
Immunoglobulin M and G (IgM and IgG) responses were followed up to 6 m
onths in patients with nephropathia epidemica (NE) by an enzyme-linked
immunosorbent assay (ELISA) using a recombinant Puumala virus (PUU) n
ucleocapsid protein as antigen and an immunofluorescence test (IF) usi
ng PUU infected, acetone-treated cells as antigen. The recombinant pro
tein was produced by cloning and expressing the nucleocapsid encoding
gene of PUU as a polyhistidine fusion protein in Escherichia coli. The
product was purified over a metal chelating ion affinity column. On a
dmission, all 17 patients had an IgM response by both methods. The IgM
titers decreased significantly by both methods 3 months after onset (
ELISA P < 0.05 and IF P < 0.05). Four of six still had detectable IgM,
however at low levels, after 6 months. Presence of specific IgG diffe
red significantly on admission between the two methods: by ELISA 8 of
17 had detectable specific IgG, whereas by IF 15 of 17 had specific Ig
G (P < 0.02). There were 10 significant titer rises between acute and
convalescent serum samples in the same patients by both methods. It is
concluded that the IgG antibody response differs in the early phase o
f NE as measured by a method using a recombinant PUU nucleocapsid prot
ein and a method using PUU infected acetone-treated cells as antigens.
Furthermore, the results suggest that it is of importance to rely on
specific IgM for serodiagnosis of NE during the acute phase. (C) 1995
Wiley-Liss, Inc.