IDENTIFICATION AND CHARACTERIZATION OF A HUMAN CDNA HOMOLOGOUS TO YEAST SK12

A monoclonal antibody, 170A1, which recognizes a nucleolar peptide of molecular weight 90,000, was raised. The protein was conserved among v arious vertebrates. To characterize the antigen, we screened a human f etal liver expression library using the monoclonal antibody as a probe . Molecular analyses of immunopositive clones suggested the presence o f a novel cDNA. It appeared to be a single-copy gene and encoded about 4- and 5-kb mRNAs. The gene appeared to be expressed in every cell te sted so far. Its deduced amino acid sequence revealed an overall homol ogy to recently described yeast SKI2. The SKI2 gene of Saccharomyces c erevisiae encoded a nucleolar protein that is involved in the antivira l system, We report here the partial human cDNA sequence and the local ization of the corresponding gene on chromosome 6p21. (C) 1995 Academi c Press, Inc.