RAT KARYOTYPING BY FLUORESCENCE IN-SITU HYBRIDIZATION (FISH) - LOCALIZATION OF ONCOGENE C-RAF TO 4Q42, RETINOBLASTOMA ANTIONCOGENE TO 15Q12, AND MITOCHONDRIAL D-LOOP-LIKE SEQUENCES TO THE Y-CHROMOSOME

Fluorescence in situ hybridization (FISH) has been used to karyotype t he rat genome with long interspersed repetitive elements (LINEs). Two- color FISH experiments were used to localize the rat c-raf oncogene to 4q42 and the rat retinoblastoma anti-oncogene to 15q12. In addition, sequences similar to the rat mitochondrial origin of replication (D-lo op-like sequences) have been found to be concentrated among repetitive element sequences on the Y chromosome. This report extends hybridizat ion-based karyotype technology to the rat, thus facilitating the appli cation of FISH to rat gene mapping. (C) 1995 Academic Press, Inc.