Aims-To determine the current bacteriology of lacrimal duct obstructio
n (LDO) and to relate the bacteriological findings to the type of symp
toms. Methods-127 samples were obtained from the lacrimal sac in 118 c
onsecutive adult patients with LDO, including nine bilateral cases. Re
sults-Altogether, 156 isolates were recovered from the 127 samples cul
tured. Cultures were positive from 84% of the samples. Gram positive b
acteria were isolated in 79 (62%) samples. The most frequently culture
d bacterial species was Staphylococcus epidermidis, representing 27% o
f the isolates. Gram negative bacteria were recovered from 26 (20%) sa
mples, and these bacteria were statistically significantly more common
in cases with copious discharge than in cases with minor discharge (p
= 0.000). Cases with simple stenosis of the lacrimal duct (SSLD) show
ed significantly less Streptococcus sp (p = 0.004) and Gram negative o
rganisms (p = 0.004) than those with chronic dacryocystitis. Conclusio
n-The bacteriology of SSLD resembles that of normal conjunctival flora
. Chronic dacryocystitis in adults is associated with an increased pro
portion of Gram negative bacteria which may be a reservoir for postope
rative intraocular infection. They should also be taken into account i
n selecting antimicrobial prophylaxis in lacrimal drainage surgery.