ADRIAMYCIN-FE3-INDUCED INACTIVATION OF ENZYMES IN ERYTHROCYTE-MEMBRANES DURING LIPID-PEROXIDATION()

Adriamycin (AD)-Fe3+ caused the inactivation of Na+-, K+-ATPase and Ca 2+-ATPase of erythrocyte membranes during lipid peroxidation. AD-Fe3also induced the formation of fluorescent substances from the membrane s with lipid peroxidation. The fluorescent substances were little extr acted by chloroform-methanol, indicating that they were retained in th e membranes. Butylated hydroxytoluene and trolox strongly inhibited bo th the inactivation of these ATPases and the formation of fluorescent substances with lipid peroxidation. Another antioxidant, vitamin E, sl ightly prevented the damage of the membranes. However, p-nitrophenyl p hosphatase activity and acetylcholine esterase have lower or no suscep tibility to the membrane lipid peroxidation. These results indicated t hat the ATPases were very sensitive to lipid peroxidation and that the membranes were modified during the peroxidation reaction.