PROTON AND DEUTERON SPIN TARGETS IN BIOLOGICAL STRUCTURE RESEARCH

Authors
STUHRMANN HB BURKHARDT N DIETRICH G JUNEMANN R MEERWINCK W SCHMITT M WADZACK J WILLUMEIT R ZHAO J NIERHAUS KH
Citation
Hb. Stuhrmann et al., PROTON AND DEUTERON SPIN TARGETS IN BIOLOGICAL STRUCTURE RESEARCH, Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment, 356(1), 1995, pp. 124-132
Citations number
29
Categorie Soggetti
Nuclear Sciences & Tecnology","Physics, Particles & Fields","Instument & Instrumentation",Spectroscopy
Journal title
Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment → ACNP
ISSN journal
01689002
Volume
356
Issue
1
Year of publication
1995
Pages
124 - 132
Database
ISI
SICI code
0168-9002(1995)356:1<124:PADSTI>2.0.ZU;2-6
Abstract
Polarized neutron scattering from polarized nuclear spin targets has b een used to study the in situ structure of ribosomal components in sol utions. The progress of this technique is substantiated by first resul ts obtained from protonated tRNA and mRNA labels in a deuterated ribos ome. The site of the tRNA could be determined uniquely, whereas there are several orientations of the [tRNA](2)-mRNA complex which are compa tible with the neutron scattering data. Similarly, a small, protonated mRNA fragment was studied. Its size favours a complicated path of the mRNA chain through the ribosome. The role of deuteron spin targets is discussed.