GENE CONTROLLED BY PROMOTER - P-TH4 DEPENDING ON WHIG OF STREPTOMYCES-COELICOLOR

The downstream gene controlled by promoter-P-TH4 which is related to S treptomyces differentiation was cloned, and its sequence was determine d by the dideoxy chain termination method. The results indicated that the 1597bp of DNA fragment conferred a complete open reading frame (OR F). In searches of databases, the deduced product of the ORF was not h omologous with any known proteins; it may be a new protein. The functi on of the gene was studied using the strategy of gene disruption; the actinorhodin could not be produced when this gene was disrupted. There fore, this gene may be related to actinorhodin biosynthesis in Strepto myces coelicolor, and the result also shows that this gene may play a role in multiple level regulation of differentiation genes in Streptom yces.