CRYSTAL-STRUCTURE OF STREPTOMYCES-DIASTATICUS NO-7 STRAIN M1033 XYLOSE ISOMERASE

The crystal structures of Streptomyces diastaticus No. 7 strain M1033 xylose isomerase (SDXyI) have been analysed and refined at 0.19 nm. Th e crystal space group is I222, with unit cell dimensions of a = 9.884 nm, b = 9.393 nm and c = 8.798 nm. Based on the coordinates of the Str eptomyces rubiginosus xylose isomerase (SRXyI), the initial model of S DXyI was built up by the close packing analysing and R-factor searchin g and refined by PROLSQ to a final R-factor of 0.177 with the rms devi ations of bond lengths and bond angles of 0.0019 nm and 2.1 degrees, r espectively. No significant global conformation change existed between SRXyI and SDXyI except the local conformation in the active site.