I. Gavidia et al., SELECTION AND LONG-TERM CULTURES OF HIGH-YIELDING DIGITALIS-OBSCURA PLANTS - RAPD MARKERS FOR ANALYSIS OF GENETIC STABILITY, PLANT SCI, 121(2), 1996, pp. 197-205
Fifteen wild-growing plants of Digitalis obscura collected in three di
fferents regions were characterized according to their capacities to b
iosynthesize cardenolides and to proliferate in vitro. Great genotype-
dependent variabilities were found in both parameters. Selected genoty
pes were differentiated using randomly amplified polymorphic DNA (RAPD
) markers. Once an elite genotype with high-yielding properties was is
olated and micropropagated during 2 years, identity of the donor plant
and the regenerants was confirmed by RAPD analysis. Copyright (C) 199
6 Elsevier Science Ireland Ltd