ROLE OF NEUROPEPTIDE-Y PROJECTION ON THE DEVELOPMENT OF SEROTONERGIC INNERVATION IN THE SUPRACHIASMATIC NUCLEUS OF THE RAT, SHOWN BY TRIPLEINTRAOCULAR GRAFTS
S. Ueda et al., ROLE OF NEUROPEPTIDE-Y PROJECTION ON THE DEVELOPMENT OF SEROTONERGIC INNERVATION IN THE SUPRACHIASMATIC NUCLEUS OF THE RAT, SHOWN BY TRIPLEINTRAOCULAR GRAFTS, Brain research, 673(2), 1995, pp. 325-330
In our previous paper, the intraocular double grafts of fetal mesencep
halic raphe and suprachiasmatic nucleus (SCN) demonstrated that the se
rotonergic fibers from raphe tissue did not show a dense innervation o
f SCN [28]. To examine the influence of NPY innervation from lateral g
eniculate nucleus (LGN) on the development of serotonergic fibers in t
he SCN, fetal mesencephalic raphe, SCN and LGN tissues were transplant
ed together into the eye chamber of adult rat. 6 weeks after transplan
tation, triple grafts were immunohistochemically examined. The SCN cel
l cluster was recognized by vasoactive intestinal polypeptide (VIP)- a
nd arginine vasopressin (AVP)-immunoreactive neurons and fibers. The S
CN cell cluster also contained a large number of serotonin-immunoreact
ive fibers from raphe tissue and a moderate number of neuropeptide Y (
NPY)-immunoreactive fibers from LGN tissue. The present results provid
e information on possible NPY-serotonin interactions in the developing
SCN.