ENZYMATIC-SYNTHESIS OF PEPTIDES OF ARGINI NE - CHROMOPHORE SUBSTRATESOF METALLOPROTEINASES AND CARBOPEPTIDASES

Subtilisin 72 sorbed on the surface of macroporous glass catalyzes a c ondensation of the esters of N-acylated peptides with arginine derivat ives in organic solvents. The sorbed enzyme can be used repeatedly, wh ich makes it possible to synthesize the chromophore substrates of meta lloproteinases and carbopeptidases of the general formula Dnp-Ala-Ala- Xaa-Arg-NH2 (Xaa = Leu, Phe, Val, Ile). In tetrapeptides, metalloprote inases hydrolyze the Ala-Xaa bond with the removal of Dnp-Ala-Ala-OH, which can be determined spectrophotometrically. The chromophore substr ates of;carboxypeptidases of the B type (Dnp-Ala-Ala-Xaa-Arg-OH and Dn p-Ala-Ala-Arg-OH) are obtained by hydrolysis of the corresponding amid es by trypsin.