3'-(TETRAZOLE-2''-YL)-3'-DEOXYTHYMIDINE A ND ITS 5''-SUBSTITUTED - SYNTHESIS AND CONFORMATION IN CRYSTAL-STATE - SUBSTRATE PROPERTIES OF 3'-(TETRAZOLE-2''-YL)-3'-DEOXYTHYMIDINE 5'-TRIPHOSPHATE TOWARDS DNA-POLYMERASES

Authors
OSTROVSKII VA STUDENTSOV EP POPLAVSKII VS IVANOVA NV GURSKAYA GV ZAVODNIK VE JASKO MV SEMIZAROV DG
Citation
Va. Ostrovskii et al., 3'-(TETRAZOLE-2''-YL)-3'-DEOXYTHYMIDINE A ND ITS 5''-SUBSTITUTED - SYNTHESIS AND CONFORMATION IN CRYSTAL-STATE - SUBSTRATE PROPERTIES OF 3'-(TETRAZOLE-2''-YL)-3'-DEOXYTHYMIDINE 5'-TRIPHOSPHATE TOWARDS DNA-POLYMERASES, Bioorganiceskaa himia, 21(1), 1995, pp. 49-54
Citations number
16
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Bioorganiceskaa himia → ACNP
ISSN journal
01323423
Volume
21
Issue
1
Year of publication
1995
Pages
49 - 54
Database
ISI
SICI code
0132-3423(1995)21:1<49:3ANI5->2.0.ZU;2-P
Abstract
5 ''-Derivatives of 3'-(tetrazole-2 ''-yl)-3'-deoxythymidines were syn thesized by interaction of 5'-benzoyl-2',3'-anhydrothymidine with tetr azole or its 5-derivatives followed by debenzoylation. Structures of t wo of them, 3'-(tetrazole-2 ''-yl)-3'-deoxythymidine and 3'-(5 ''-meth yltetrazole-2 ''-yl)-3'-deoxythymidine, elucidated by X-ray analysis, revealed anti conformation of thymine about the glycosidic bond and 2' -endo-3'-exo-conformation of the sugar residue with gauche(+) orientat ion with respect to C4'-C5'-bond. 3'-(Tetrazole-2 ''-yl)3'-deoxythymid ine 5'-triphosphate demonstrated poor substrate properties for the avi an myeloblastosis virus reverse transcriptase and none for several oth er DNA polymerases.