CLOSTRIDIUM CELLULOLYTICUM VIABILITY AND SPORULATION UNDER CELLOBIOSESTARVATION CONDITIONS

Citation
A. Gehin et al., CLOSTRIDIUM CELLULOLYTICUM VIABILITY AND SPORULATION UNDER CELLOBIOSESTARVATION CONDITIONS, Applied and environmental microbiology, 61(3), 1995, pp. 868-871
Citations number
21
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
00992240
Volume
61
Issue
3
Year of publication
1995
Pages
868 - 871
Database
ISI
SICI code
0099-2240(1995)61:3<868:CCVASU>2.0.ZU;2-7
Abstract
Depending on the moment of cellobiose starvation, Clostridium cellulol yticum cells behave in different ways, Cells starved during the expone ntial phase of growth sporulate at 30%, whereas exhaustion of the carb on substrate at the beginning of growth does not provoke cell sporulat ion, Growth in the presence of excess cellobiose generates 3% spores. The response of C. cellulolyticum to carbon starvation involves change s in proteolytic activities; higher activities (20% protein degradatio n) corresponded to a higher level of sporulation; lower proteolysis (5 %) was observed in cells starved during the beginning of exponential g rowth, when sporulation was not observed; with an excess of cellobiose , an intermediate value (10%), accompanied by a low level of sporulati on, was observed in cells taken at the end of the exponential growth p hase. The basal percentage of the protein breakdown in nonstarved cult ure was 4%. Cells lacking proteolytic activities failed to induce spor ulation. High concentrations of cellobiose repressed proteolytic activ ities and sporulation. The onset of carbon starvation during the growt h phase affected the survival response of C. cellulolyticum via the sp orulation process and also via cell-cellulose interaction, Cells from the exponential growth phase were more adhesive to filter paper than c ells from the stationary growth phase but less than cells from the lat e stationary growth phase.