L. Watrin et al., MINIMAL AMINO-ACID-REQUIREMENTS OF THE HYPERTHERMOPHILIC ARCHAEON PYROCOCCUS-ABYSSI, ISOLATED FROM DEEP-SEA HYDROTHERMAL VENTS, Applied and environmental microbiology, 61(3), 1995, pp. 1138-1140
A minimal growth medium containing only nine amino acids and vitamins
as the sole carbon and energy sources allowed the growth of Pyrococcus
abyssi GE 5, a novel hyperthermophilic sulfur-metabolizing archaeon i
solated from deep-sea hydrothermal vents. The generation time in this
medium was about 40 min, and cell densities up to 5 x 10(8) cells ml(-
1) were attained. These results are similar to those obtained previous
ly with complex proteinaceous media.