MULTIPLE VIBRIO-VULNIFICUS STRAINS IN OYSTERS AS DEMONSTRATED BY CLAMPED HOMOGENEOUS ELECTRIC-FIELD GEL-ELECTROPHORESIS

Authors
BUCHRIESER C GANGAR VV MURPHREE RL TAMPLIN ML KASPAR CW
Citation
C. Buchrieser et al., MULTIPLE VIBRIO-VULNIFICUS STRAINS IN OYSTERS AS DEMONSTRATED BY CLAMPED HOMOGENEOUS ELECTRIC-FIELD GEL-ELECTROPHORESIS, Applied and environmental microbiology, 61(3), 1995, pp. 1163-1168
Citations number
30
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
61
Issue
3
Year of publication
1995
Pages
1163 - 1168
Database
ISI
SICI code
0099-2240(1995)61:3<1163:MVSIOA>2.0.ZU;2-A
Abstract
Clamped homogeneous electric field gel electrophoresis and a computer program for managing electrophoresis handing patterns (ELBAMAP) were u sed to analyze genomic DNA of 118 Vibrio vulnificus strains, isolated from three oysters by direct plating. Analysis with SfiI resulted in 6 0 restriction endonuclease digestion profiles (REDP), while analysis w ith SrfI produced 53 different REDP. Similarities between REDP ranged from 7 to 93%. Principal-component analysis showed that the strains we re heterogeneous.