THE INTERACTION IN-VITRO OF HUMAN SPERMATOZOA WITH EPITHELIAL-CELLS FROM THE HUMAN UTERINE (FALLOPIAN) TUBE

The physical interaction between human spermatozoa and the epithelium of the human uterine (Fallopian) tube was investigated in vitro using a variety of techniques. The 'live' observation of human spermatozoa i ncubated with 1 day old cultures of tubal epithelium demonstrated that spermatozoa can show a strong physical interaction with epithelial ce lls; contact with the epithelium appeared to be random and there was n o evidence of any taxis toward epithelial cells. The physical interact ion (or 'binding') was resistant to gentle washing and was maintained following the addition of glutaraldehyde fixative. The intimate nature of the interaction was confirmed ultrastructurally where both spermat ozoa and epithelial membranes were observed to be in close apposition. These results are the first descriptions of sperm-epithelial 'binding ' in the human. They are similar to other observations made in a varie ty of non-human mammalian species. It is suggested that this interacti on may be an important feature of normal sperm transport in the human uterine tube in vivo.