CHARACTERIZATION OF THE PROMOTER REGION OF THE HUMAN APURINIC ENDONUCLEASE GENE (APE)

Apurinic/apyrimidinic (AP) sites are mutagenic and block DNA synthesis in vitro, Repair of AP sites is initiated by AP endonucleases that cl eave just 5' to the damage. We linked a 4.1-kilobase pair HindIII DNA fragment from the region upstream of the human AP endonuclease gene (A PE) to the chloramphenicol acetyltransferase (CAT) gene. Deletions gen erated constructs containing 1.9 kilobase pairs to 50 base pairs (bp) of the APE upstream region. Transient transfection studies in HeLa cel ls established that the basal APE promoter is contained within a 500-b p fragment. The major transcriptional start site in HeLa, hepatoma (He pG2), and myeloid leukemic (K562) cells was mapped to a cluster of sit es similar to 130 bp downstream of a putative ''CCAAT box,'' similar t o 130 bp 5' of the first splice junction in APE. Deletion of 5' sequen ces to within 10 bp of the CCAAT box reduced the CAT activity by only about half, and removal of the CCAAT box region left a residual promot er activity similar to 9%. Deletion to 31 bp upstream of the transcrip tional start site abolished APE promoter activity. DNA sequence analys is revealed potential transcription factor recognition sites in the AP E promoter. Gel mobility-shift assays showed that both human upstream factor and Spl can bind their respective sites in the APE promoter. Ho wever, DNase I footprinting using HeLa nuclear extract showed that the binding of Sp1 and upstream factor is blocked by the binding of other proteins to the nearby CCAAT box region.