SPERM CHROMOSOME ANALYSIS OF KARAN-SWISS AND KARAN-FRIES CROSSBRED BULLS

Zona-free hamster eggs were penetrated by Karan Swiss and Karan Fries crossbred bull spermatozoa capacitated using calcium ionophore A23187. Zona-free hamster egg penetration percentage varied between 59 and 83 among 11 crossbred bulls. Penetrated eggs were cultured in TC 199 med ium with 10% fetal calf serum. Podophyllotoxin and vinblastine were us ed to interrupt karyogamy and tubulin polymerization, respectively. Gr adual fixation air drying method was modified for the preparation of s lides. Hamster cattle metaphase yield was 28 +/- 5.2%. The analysis of 105 crossbred cattle sperm metaphases indicated that 51 contained a Y -chromosome and 54 had an X-chromosome. The incidence of aneuploidy wa s 14.28%.