SYNTHESIS AND BINDING TO DNA OF 2 PEPTIDES MODELING THE DNA-BINDING DOMAIN OF TRANSCRIPTION ACTIVATOR V-JUN

Citation
Sl. Grokhovskii et al., SYNTHESIS AND BINDING TO DNA OF 2 PEPTIDES MODELING THE DNA-BINDING DOMAIN OF TRANSCRIPTION ACTIVATOR V-JUN, Molecular biology, 28(5), 1994, pp. 730-737
Citations number
40
Categorie Soggetti
Biology
Journal title
ISSN journal
00268933
Volume
28
Issue
5
Year of publication
1994
Part
2
Pages
730 - 737
Database
ISI
SICI code
0026-8933(1994)28:5<730:SABTDO>2.0.ZU;2-8
Abstract
Synthesis and interaction with DNA of two 26-residue peptides, each co ntaining in two copies a fragment of the DNA-binding domain of the tra nscription activator v-Jun, are described. The peptides have identical amino acid sequence but one is stabilized by an S-S bond between two cysteine residues. CD spectroscopy showed that both peptides have a ra ndom conformation in an aqueous solution, and assume a partly alpha-he lical conformation in the presence of 20% trifluoroethanol. The relati ve content of alpha-helix increases in the presence of 40% trifluoroet hanol to approximately 66% and 40%, respectively in the absence and in the presence of the S-S bond. Obviously the S-S bond hinders the form ation of alpha-helix within one of the DNA-binding regions of the pept ide, though an alpha-helix can probably be generated in the other part . Both peptides were shown to bind to DNA. We revealed that the DNA mi nor groove-binding antibiotic distamycin A competes effectively with t he peptide for binding to poly(dA).poly(dT). Binding of the peptide to DNA induces a conformational transition in the peptide molecule, whil e the DNA structure does not appreciably change. The difference CD spe ctra obtained by subtracting the spectrum of free DNA from that of a p eptide-DNA mixture differs from the spectrum of the free peptide. The shapes of the difference spectra are consistent with alpha --> beta an d coil --> beta transitions induced upon binding of the peptides to DN A. DNase I footprinting diagrams show that the peptides protect the DN A from cleavage at the regions containing 5'-TGA-3' and 5'-TGC-3' sequ ences.