V. Kost et al., SUBREGIONAL LOCALIZATION OF RECOMBINANT COSMIDS CONTAINING MICROSATELLITE REPEATS ON HUMAN-CHROMOSOME-13-M, Molecular biology, 28(5), 1994, pp. 738-742
Fluorescence in situ hybridization (FISH) was used to map 24 recombina
nt cosmids on human chromosomes. Unique location was found for 15 clon
es, 13 on chromosome 13, one on chromosome 1, and one on chromosome 11
. Nine cosmids producing signals on chromosome 13 and on other chromos
omes containing ribosomal genes were related to the chromosomal nucleo
lar organizing region (NOR). Three of the 24 clones had no microsatell
ite repeats, and in 21 clones microsatellite repeats were formed by GA
CA, GACT, GATG, TCC, or CA motifs. The motifs GACA and TCC were common
for the 9 NOR-related clones. As to the 15 clones with unique signals
, the GACT motif was present in 9 clones, and GATG, TCC and CA were fo
und in one clone each. The described clones can be used to construct h
ighly polymorphic molecular genetic markers for fine genetic mapping o
f human chromosome 13.