BLOT HYBRIDIZATION OF REPEATED SEQUENCE FAMILY OF HUMPBACK SALMON (ONCORHYNCHUS-GORBUSCHA)

A family of DNA repeated sequences from Pacific salmons (genus Oncorhy nchus) with species-specific restriction endonuclease digestion patter ns was studied. The experiments were performed with Oncorhynchus gorbu scha DNA in which this DNA fraction is easily detected electrophoretic ally. Comparative analysis of blot hybridization experiments was done, in which one of the fragments obtained by restriction endonuclease di gestion of homologous or heterologous DNA was used as a hybridization probe. By comparing the electrophoretic profiles with blot hybridizati on patterns and taking into account the size of DNA fragments particip ating in the reaction, one could suggest a hypothetical structure of D NA repeated sequence family composed of scattered or clustered repeats and demonstrate the presence of units with higher degree of similarit y within such a family.