HYBRID PEPTIDE-CONTAINING RGDF (ARG-GLY-ASP-PHE) COUPLED WITH THE CARBOXY-TERMINAL PART OF ALPHA(2)-ANTIPLASMIN CAPABLE OF INHIBITING PLATELET-AGGREGATION AND PROMOTING FIBRINOLYSIS

The aim of this study was to synthesize and investigate hybrid peptide s which contain the RGD (Arg-Gly-Asp) sequence coupled with lysine res idues in special arrangements (antiplasmin carboxyterminal peptide) in an effort to simultaneously inhibit platelet aggregation and promote fibrinolysis. The in vitro haemostatic modifying properties of the syn thesized peptides were tested by ADP-induced platelet aggregation, pla smin-generation tests and fibrin-clot lysis assays. The hybrid peptide RGDFAP, composed of RGDF (Arg-Gly-Asp Phe) coupled to a synthetic pep tide residue of the carboxyterminal part of antiplasmin (AP(26)) inhib ited platelet activation and increased plasmin generation and in vitro fibrin-clot lysis.