M. Buttini et H. Boddeke, PERIPHERAL LIPOPOLYSACCHARIDE STIMULATION INDUCES INTERLEUKIN-1-BETA MESSENGER-RNA IN RAT-BRAIN MICROGLIAL CELLS, Neuroscience, 65(2), 1995, pp. 523-530
The inflammatory cytokine interleukin-1 acts as an endogenous pyrogen
in organisms affected by infectious diseases and has been shown to inf
luence the activity of the central nervous system. Using in situ hybri
dization histochemistry, we have examined the cellular source of inter
leukin-1 beta in rat brain after peripheral stimulation with the bacte
rial lipopolysaccharide, a potent inducer of interleukin-1. Whereas no
interleukin-1 beta messenger RNA could be detected in brains in unsti
mulated rats, lipopolysaccharide induced a transient, high and widespr
ead expression of interleukin-1 beta messenger RNA in the entire brain
. The highest levels of interleukin-1 beta messenger RNA were observed
6-8 h after stimulation with lipopolysaccharide. Using a combination
of non-radioactive in situ hybridization and immunocytochemistry with
the microglial-specific antibody OX-42, interleukin-1 beta messenger R
NA-positive cells could be identified as microglia. We conclude that b
rain microglial cells are the major source of interleukin-1 beta messe
nger RNA after peripheral administration of lipopolysaccharide.