With new EEC regulations, alternative treatment and disposal technique
s of the excess sludge produced by Activated Sludge (AS) wastewater tr
eatment plants have to be performed. In order to reduce the excess slu
dge produced, experiments have been carried out with a Membrane BioRea
ctor (MBR) to study the maintenance and cryptic growth phenomena of Ps
eudomonas fluorescens culture taken as a model when grown on a limitin
g substrate complex medium similar to a synthetic urban wastewater. Ex
periments with various imposed wasting rates showed that viability and
sludge production yield decreased when sludge age increased. Same var
iations were observed on the cell content ratio protein/polysaccharide
by analysis of the cell lysis products released after discontinuous t
hermal treatment. Biomass growth on these cell lysis products was achi
eved to characterize cryptic growth and its impact on sludge productio
n yield. Finally, a continuous sludge thermal treatment system was ope
rating with MBR to amplify sludge breakage and consequently biomass gr
owth on the lysis products. With the promising results obtained, this
work gives a new outlook on the AS process and leads to the developmen
t of processes with control and reduction of sludge production.