D. Wilmanska et al., IDENTIFICATION OF CHOLESTEROL OXIDASE FROM FAST-GROWING MYCOBACTERIALSTRAINS AND RHODOCOCCUS SP, Journal of fermentation and bioengineering, 79(2), 1995, pp. 119-124
Cholesterol oxidase synthesis ability by various fast-growing mycobact
eria (M. fortuitum, M. vaccae, M. phlei, M. smegmatis) and Rhodococcus
sp. IM 58 was investigated by thin-layer chromatography and Western b
lot analysis. In contrast with the case of cholesterol oxidases from A
rthrobacter sp. IM 79 and Streptomyces sp., those from the strains tes
ted were localized intracellularly. Western blot experiments revealed
high antigenic similarity of those enzymatic proteins to cholesterol o
xidase from Schizophyllum commune, and lack of homology to cholesterol
oxidase from Streptomyces sp. Differences in the analyzed enzymes wer
e due not only to a single antigenic determinant alteration but also t
o the significant distinctions on the genetic level as shown by Southe
rn blot hybridization.