KARYOLOGICAL STUDY OF ABIES-CONCOLOR X ABIES-GRANDIS CALLI AND SHOOTSREGENERATED IN-VITRO

In the present work was determined the extent of somaclonal variabilit y in adventitious and axillary shoots regenerated in vitro from mature embryos and cotyledons of seedlings and in long-term callus cultures of vital hybrid A. concolor x A. grandis. Karyological analysis was re alised by determination of chromosome numbers in mitotic cells and by flow cytometric analysis of nuclear DNA content. Our results showed th at both adventitious and axillary shoots as well as long-term callus c ultures are karyologically stable and remain at the diploid level.