OXYGEN-UPTAKE OF GERMINATING WHEAT EMBRYOS USING SALICYLHYDROXAMIC ACID (SHAM)

The titration curves of respiration rates throughout inbibition and ge rmination of isolated wheat embryos (Triticum durum, cv. Appulo) were determined. From these curves the appropriate salicylhydroxamic acid ( SHAM) concentrations needed to inhibit the cyanide-resistant alternati ve pathway, without any side effects were estimated for each stage: 5 mmol.l-1 for 30 min, 10 mmol.l-1 for 3, 6, 12 and 48 h and 15 mmol.l-1 for 24 h.