ADAPTATION OF THE TROPONIN-T ELISA TEST TO A MICROPLATE IMMUNOASSAY READER

Troponin T concentration in serum is usually measured by the automated method developed by Boehringer Mannheim for the ES-series of analyser s. These instruments need at least 140 mu l of serum and 700 mu l of r eagents for a single analysis, which takes 90 min. We describe an alte rnative procedure, using streptavidin-coated microtitre plates, tropon in T reagents of Boehringer Mannheim and an ELISA-reader to measure th e concentration of troponin T. The present assay needs only 30 mu l of sample and 200 mu l of reagents, and it takes 75 min; the detection l imit is 0.10 mu g/l. We also assessed the microtitre plate method for sensitivity and precision and compared the results with those measured by an ES-300 automatic analyser. Both methods have the same measureme nt range for troponin T of 0.1 to 15 mu g/l. For daily routine use of the microtitre plate method we recommend duplicate determinations.