W. Nieuwenhuizen et al., A ONE-STEP ENZYME-IMMUNOASSAY FOR TOTAL PLASMINOGEN-ACTIVATOR INHIBITOR-1 ANTIGEN IN HUMAN PLASMA, Blood coagulation & fibrinolysis, 6(3), 1995, pp. 268-272
A one-step enzyme immunoassay (EIA) has been developed for plasminogen
activator inhibitor-1 (PAI-1) antigen. The assay is based on polyclon
al antibodies, which were found to be slightly more reactive with tiss
ue-type plasminogen activator (t-PA)/PAI-1 complexes and latent PAI-1
than with active PAI-1. To correct this, active PAI-1 is converted to
t-PA/PAI-1 complexes. Latent PAI-1 and t-PA/PAI-1 complexes were equal
ly reactive. The EIA is specific (PAI-2 and PAI-3 are not detected), p
recise (CVs range from 1.8% to 11.1%, depending on the PAI-1 concentra
tion), fast (assay time less than 3 h), and easy to perform. It is com
patible with the use of Stabilyte(R) plasma. The assay is calibrated a
gainst the putative international standard of the National Institute o
f Biological Standards and Control (NIBSC; lot 87/512). The normal ran
ges found with this EIA were 13.2-88 ng/ml; one apparently normal dono
r had a value of 100 ng/ml.