DEFIBRINATION ESSENTIAL IN THE ASSAY OF IONIZED MAGNESIUM IN MONONUCLEAR-CELLS

Authors
WILLEMS HL KRUSE J GEVEN WB VIS P MONNENS LAH
Citation
Hl. Willems et al., DEFIBRINATION ESSENTIAL IN THE ASSAY OF IONIZED MAGNESIUM IN MONONUCLEAR-CELLS, European journal of clinical chemistry and clinical biochemistry, 33(2), 1995, pp. 103-104
Citations number
7
Categorie Soggetti
Biology,"Chemistry Medicinal
Journal title
European journal of clinical chemistry and clinical biochemistry → ACNP
ISSN journal
09394974
Volume
33
Issue
2
Year of publication
1995
Pages
103 - 104
Database
ISI
SICI code
0939-4974(1995)33:2<103:DEITAO>2.0.ZU;2-K
Abstract
Intracellular free (ionized) magnesium concentration was measured in m ononuclear cells isolated from healthy volunteers by use of dual wavel ength fluorescence (indicator. mag-fura-2). We found a free Mg2+ conce ntration of 1.28 +/- 0.08 mmol/l in mononuclear cells isolated from he parinized blood. When we defibrinated blood samples prior to the isola tion step we measured 0.78 +/- 0.05 mmol/l of free Mg2+ in these cells . We conclude that this difference is caused by the platelets present in the heparinized specimens.