PLATELET-AGGREGATION INHIBITING AND ANTICOAGULANT EFFECTS OF OLIGOAMINES .29. INFLUENCE OF THE ANTITHROMBOTIC OLIGOAMINE RE-1492 ON THE STORAGE OF RED-BLOOD-CELLS, MORPHOLOGY, THEIR POTASSIUM AND ATP CONTENT, HEMOLYSIS AND VIABILITY

The influence of RE 1492 on the storage of red blood cells (RBCs) was investigated. RE 1492 (c greater than or equal to 50 mu mol/L) induces the formation of cup cells and hence delays the appearance of echinoc ytes and crenated spheres in the blood. A concentration of 50 mu mol/L RE 1492 drops hemolysis by 50 %. At this concentration after 6 h of s torage the potassium efflux was enhanced significantly. The ATP concen tration was decreased between 12 h and 24 h of storage. During the fir st 6 h these alterations have no effect on the in vivo viability of RB Cs. After 12 h of storage a significant reduction in viability is obse rved, We suppose that the changes observed are due to the intercalatio n of RE 1492 in the phospholipid matrix of the RBC membrane, followed by an accelerated substrate turnover in the RBCs.