Jq. Wu et J. Pawliszyn, APPLICATION OF CAPILLARY ISOELECTRIC-FOCUSING WITH ABSORPTION IMAGINGDETECTION TO THE QUANTITATIVE-DETERMINATION OF HUMAN HEMOGLOBIN-VARIANTS, Electrophoresis, 16(4), 1995, pp. 670-673
The capillary isoelectric focusing (CIEF)-absorption imaging detector
is a multicapillary separation instrument. Several protein samples can
be separated and detected in a single run. The analysis time for one
run is only 3 min. Because an imaging detector is used, the mobilizati
on process of conventional CIEF is not necessary, eliminating such dra
wbacks as long analysis time and poor reproducibility in mobilization
speed. Human hemoglobin variants were quantitatively determined using
the instrument. The peak areas of the analytes were proportional to th
eir concentrations in the concentration range of 0-200 mu g/mL. Hemogl
obin variant A(2). which only comprises 2% of the whole hemoglobin, ca
n be quantitatively determined with a standard deviation of less than
10%. For the high concentration variants, such as variant S, the devia
tion is less than 1%.