IMMUNOHISTOCHEMICAL DEMONSTRATION OF 4 SUBUNITS OF NEUTROPHIL NAD(P)HOXIDASE IN TYPE-I CELLS OF CAROTID-BODY

We demonstrate, by means of immunohistochemistry, that type I cells of human, guinea pig, and rat carotid bodies react with antisera raised against the subunits p22(phox), gp91(phox), p47(phox), and p67(phox), of the NAD(P)H oxidase isolated from human neutrophil granulocytes. Th e findings support previous photometric studies that indicate that car otid body type I cells possess a putative oxygen sensor protein that i s similar to the neutrophil NAD(P)H oxidase and consists of a hydrogen peroxide generating low-potential cytochrome b(558) With cofactors re gulating the electron transfer to oxygen.