DEMONSTRATION OF THE PHILADELPHIA TRANSLOCATION BY FLUORESCENCE IN-SITU HYBRIDIZATION (FISH) IN PARAFFIN SECTIONS AND IDENTIFICATION OF ABERRANT CELLS BY A COMBINED FISH IMMUNOPHENOTYPING APPROACH

Authors
NOLTE M WERNER M EWIG M VONWASIELEWSKI R WILKENS L GEORGII A
Citation
M. Nolte et al., DEMONSTRATION OF THE PHILADELPHIA TRANSLOCATION BY FLUORESCENCE IN-SITU HYBRIDIZATION (FISH) IN PARAFFIN SECTIONS AND IDENTIFICATION OF ABERRANT CELLS BY A COMBINED FISH IMMUNOPHENOTYPING APPROACH, Histopathology, 26(5), 1995, pp. 433-437
Citations number
22
Categorie Soggetti
Cell Biology",Pathology
Journal title
Histopathology → ACNP
ISSN journal
03090167
Volume
26
Issue
5
Year of publication
1995
Pages
433 - 437
Database
ISI
SICI code
0309-0167(1995)26:5<433:DOTPTB>2.0.ZU;2-0
Abstract
The Philadelphia translocation was demonstrated by two-colour fluoresc ence in situ hybridization (FISH) in decalcified paraffin sections of bone marrow from patients with chronic myelogenous leukaemia, FISH was combined with immunocytochemical detection of different membrane-boun d or cytoplasmic antigens. With this new technique, the cells bearing the 9;22 translocation can be identified morphologically, as well as i mmunocytochemically, in tissue sections.