DESIGN NOTE - A PERSONAL-COMPUTER-BASED THERMOCYCLER FOR POLYMERASE CHAIN-REACTIONS

Authors
UPADHYAY PK GADRE DV
Citation
Pk. Upadhyay et Dv. Gadre, DESIGN NOTE - A PERSONAL-COMPUTER-BASED THERMOCYCLER FOR POLYMERASE CHAIN-REACTIONS, Measurement science & technology, 6(5), 1995, pp. 588-592
Citations number
10
Categorie Soggetti
Instument & Instrumentation",Engineering
Journal title
Measurement science & technology → ACNP
ISSN journal
09570233
Volume
6
Issue
5
Year of publication
1995
Pages
588 - 592
Database
ISI
SICI code
0957-0233(1995)6:5<588:DN-APT>2.0.ZU;2-#
Abstract
A Pc-based thermocycler for polymerase chain reactions is described. I t uses electric heaters and 4 degrees C water for heating and cooling respectively. The temperature control is built around the easily acces sible parallel printer adaptor of an IBM-compatible pc. An Ic temperat ure transducer LM335 is used for sensing the temperature. Pulse width modulation of the Ac mains is used to control the energy output to the heaters. The cost of the set-up, excluding a pc and a chiller, is aro und US$100. The thermocycler was tested in amplifying DNA in actual la boratory situations.