Ld. Payne et al., DETERMINATION OF ABAMECTIN AND OR IVERMECTIN IN CATTLE FECES AT LOW PARTS-PER-BILLION LEVELS USING HPLC WITH FLUORESCENCE DETECTION/, Journal of agricultural and food chemistry, 43(5), 1995, pp. 1233-1237
Abamectin and ivermectin are potent antiparasitic animal health drugs
that differ structurally by a double bond. Abamectin is a natural prod
uct produced by the soil microorganism Streptomyces avermitilis. Iverm
ectin is a unique hydrogenation product of abamectin. A sensitive HPLC
-fluorescence method was developed to determine abamectin or ivermecti
n residues in bovine feces. The limit of quantitation is 2 ppb (ng/g)
and the limit of detection is 1 ppb (wet weight basis). The range vali
dated was from 2 ppb to 2 ppm (mu g/g) with average recoveries of 84%
for both abamectin and ivermectin. Abamectin and ivermectin can be ana
lyzed simultaneously because the fluorescent derivatives of the two co
mpounds are chromatographically well resolved on the C-18 column used.
The method was developed to support studies concerning the dissipatio
n of incurred abamectin or ivermectin residues in cattle feces postdos
e and postdeposition.